ISB News

Tuberculosis Research: A ‘Molecular Road Map’ to Help Understand Gene Regulation

The journal “Trends in Microbiology” recently published a spotlight article on a tuberculosis research collaboration between scientists at Institute for Systems Biology and Seattle BioMed. The paper “The DNA-binding network of Mycobacterium tuberculosis” was published in the journal “Nature Communications” (Jan. 12, 2015):

“MTB employs about 200 different molecular switches to sense and respond to the shifting, hostile landscape of the host. To identify and understand the intertwining gene regulatory network that the molecular switches control, Seattle BioMed and ISB researchers (from the Baliga and Price Labs) examined the consequences of overexpressing (or boosting the function of) each switch. The team overexpressed almost every regulatory gene in MTB, measured the consequences of each perturbation, and used the results to identify genome-wide regulatory interactions for MTB. These results were assembled into an easily searchable map and will stimulate additional systems-level and hypothesis-driven efforts to understand MTB adaptations that promote disease.” Read more…

The spotlight article in “Trends in Microbiology” describes the “remarkable” comprehensiveness of the genome-wide characterization of transcription factor binding sites and highlighted ISB’s Baliga Lab:

Baliga and coworkers have reconstructed a comprehensive gene regulatory network consisting of 3,922 genes, covering 98 percent of the genome by using more than 2,000 transcriptome datasets in different conditions and integrating protein–protein influences as well as operon associations derived from different computational methods.” Read more…

 

Related Articles

  • Path-seq illustration

    Profiling Pathogen Gene Expression from Infected Host Cells

    Researchers at ISB reported a novel method, Path-seq, to profile expression of all MTB genes within infected mice. This study presents the most comprehensive transcriptome profiling of MTB from in vivo infection and a major technical advancement for studying any host-pathogen interaction.

    Read More
  • Biology students

    Biology Classes ‘Come Alive’ After Teacher’s Summer with ISB

    Kyle Kinzler, a high school biology teacher from Portland, Oregon, spent part of last summer at Institute for Systems Biology to evolve his curriculum and learn new ways to teach relevant, compelling and innovative content to his students. As a result, he says his classes has “come alive.”

    Read More
  • Spotlight on Amy Zamora, ISB Systems Research Scholar

    Amy Zamora joined ISB in August as a Systems Research Scholar. The Systems Research Scholars Program provides recent college undergraduates a springboard to become the next generation’s pioneers of interdisciplinary scientific research. In this Q&A, Zamora describes her experiences at ISB, research interests, future aspirations, and much more.

    Read More
Show More Articles